Barker S B & Summerson W H. The colorimetric determination of lactic acid in biological material. J. Biol. Chem. 138:535-54, 1941

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چکیده

This procedure achieved the sensitive and specific quantitation of lactate in biological fluids, eliminating the previous need for oxidation, distillation, and titration. Heating in concentrated sulfuric acid produced acetaldehyde which was directly determined by the purple color formed with p-hydroxydiphenyl in the presence of cupric copper. [The SCI® indicates that this paper has been explicitly cited in over 2,500 publications since 1961. Of these, 70 were in 1980, 70 in 1981, and 55 in 1982.)

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Association of Official Agricultural Chemists (1945). Official and Tentative Method8 of Analy8i8, 6th ed. Washington, D.C. Barker, S. B. & Summerson, W. H. (1941). J. biol. Chem. 138, 535. Dougherty, R. W. (1941). J. Amer. vet. med. A88. 99, 110. Elsden, S. R. (1945-6). J. exp. Biol. 22, 51. Elsden, S. R. & Phillipson, A. T. (1948). Ann. Rev. Biochem. 17, 705. Friedemann, T. E. (1938). J. biol....

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تاریخ انتشار 2002