Barker S B & Summerson W H. The colorimetric determination of lactic acid in biological material. J. Biol. Chem. 138:535-54, 1941
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چکیده
This procedure achieved the sensitive and specific quantitation of lactate in biological fluids, eliminating the previous need for oxidation, distillation, and titration. Heating in concentrated sulfuric acid produced acetaldehyde which was directly determined by the purple color formed with p-hydroxydiphenyl in the presence of cupric copper. [The SCI® indicates that this paper has been explicitly cited in over 2,500 publications since 1961. Of these, 70 were in 1980, 70 in 1981, and 55 in 1982.)
منابع مشابه
Ranunculin; the precursor of the vesicant substance of the buttercup.
Association of Official Agricultural Chemists (1945). Official and Tentative Method8 of Analy8i8, 6th ed. Washington, D.C. Barker, S. B. & Summerson, W. H. (1941). J. biol. Chem. 138, 535. Dougherty, R. W. (1941). J. Amer. vet. med. A88. 99, 110. Elsden, S. R. (1945-6). J. exp. Biol. 22, 51. Elsden, S. R. & Phillipson, A. T. (1948). Ann. Rev. Biochem. 17, 705. Friedemann, T. E. (1938). J. biol....
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Hilditch, T. P. (1947). The Chemical Con8titution of Natural Fats, 2nd ed. pp. 306-310. London: Chapman and Hall, Ltd. Howard, G. A. & Martin, A. J.P. (1950). Bsochem. J. 44, 532. Hunter, G. D. & Popjak, G. (1951). Biochem. J. 48, v. Klem, A. (1943). Nor8ke Vid. Akad. no. 27. MoClymont, G. L. (1949). Biochem. J. 45, i. Martin, A. J. P. (1950). Unpublished observations. Peters, J. P. & Van Slyke...
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Cohen, J. A., Oosterbaan, R. A., Warringa, M. G. P. J. & Jansz, H. S. (1955). Disc. Faraday Soc. 20, 114. Crumpler, H. R. & Dent, C. E. (1949). Nature, Lond., 164, 441. Doherty, D. G. & Vaslow, F. (1952). J. Amer. chem. Soc. 74, 931. Epstein, J., Rosenblatt, D. H. & Demek, M. M. (1956). J. Amer. chem. Soc. 78, 341. Folin, 0. & Ciocalteu, V. (1927). J. biol. Chem. 73, 627. Jandorf, B. J., Michel...
متن کاملThe colorimetric determination of lactic acid in silage.
The fundamental reaction which takes place in the process of ensilage is the conversion of a proportion of the soluble carbohydrates present to lactic acid. This reaction is of such importance that when material of high protein content is being ensiled, it is desirable to add molasses to provide a readily available substrate for the lactobacilli which occur along with coliform organisms in the ...
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تاریخ انتشار 2002